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TSKgel PWXL


TSKgel PWXL Columns for Analysis of Water-Soluble Polymers

TSKgel PWXL columns are composed of spherical, hydrophilic polymethacrylate beads. The main application area for TSKgel PWXL columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. See the table on the TSKgel PW page for typical samples and suitable eluents.

Figure 1 shows the calibration curves for all TSKgel PWXL-type columns. Figure 2 shows the benefits of TSKgel PWXL columns over TSKgel PW columns, namely the improved resolution due to the smaller particle sizes used in TSKgel PWXL columns.


Figure 1: Polyethylene glycol and oxide 
calibration curves on TSKgel PWXL columns  


 
Figure 2: Faster analysis and higher resolution with TSKgel PWXL columns.


                               




Although proteins can be analyzed on TSKgel PWXL columns, pore volumes are much smaller than on TSKgel SW-type columns. Figure 3 demonstrates the limited separation power of TSKgel PWXL for protein samples.


Specialty Columns

TSKgel G-DNA-PW is available for the analysis of DNA fragments. Similarly, TSKgel G-Oligo-PW is the preferred column for the analysis of oligosaccharides. 

The mixed-mode column, TSKgel GMPWXL, has an extended linear calibration range, suitable for samples with a broad MW distribution and unknowns.

A smaller particle size version of the popular TSKgel G-Oligo-PW column for oligomer separation is now also available. By using 3 micron instead of 7 micron particles a separation performed on an TSKgel G-Oligo-PW column can be done in half the time on a TSKgel SuperOligoPW column.

                                                                                                                                                                                                                                                           
Figure 3: Protein Calibration curves for TSKgel PWXL

Base Material: hydroxylated polymethacylate
Particle Size (mean): 7 µm
Pore size (mean): < 20 nm
Calibration Range (polyethylene glycols and oxides):   < 3,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housingmaterial ID (mm) Length (cm)
0008020
TSKgel G2500PWXL 7 µm Stainless Steel 7.8 30
0008033
Guard Column for P/N 0008020
12 µm
Stainless Steel 6 4
0008035
TSKgel Top-Off for PWXL, 1g 10 µm


Base Material: hydroxylated polymethacylate
Particle Size (mean): 7 µm
Pore size (mean): 20 nm
Calibration Range (polyethylene glycols and oxides):   < 40,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0008021
TSKgel G3000PWXL
7 µm
Stainless Steel 7.8 30
0008033
Guard Column for P/N 0008021
12 µm
Stainless Steel 6 4
0008035
TSKgel Top-Off for PWXL, 1g
10 µm
Base Material: hydroxylated polymethacylate
Particle Size (mean): 10 µm
Pore size (mean): < 50 nm
Calibration Range (polyethylene glycols and oxides):   2,000 - 300,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0008022
TSKgel G4000PWXL
10 µm
Stainless Steel 7.8 30
0008033
Guard Column for P/N 0008022
12 µm
Stainless Steel 6 4
0008035
TSKgel Top-Off for PWXL, 1g
10 µm
Base Material: hydroxylated polymethacylate
Particle Size (mean): 10 µm
Pore size (mean): 100 nm
Calibration Range (polyethylene glycols and oxides):   4,000 - 800,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0008023
TSKgel G5000PWXL
10 µm
Stainless Steel 7.8 30
0008033
Guard Column for P/N 0008023
12 µm
Stainless Steel 6 4
0008035
TSKgel Top-Off for PWXL, 1g
10 µm
Base Material: hydroxylated polymethacylate
Particle Size (mean): 13 µm
Pore size (mean): > 100 nm
Calibration Range (polyethylene glycols and oxides):   40,000 - 8,000,000 Da
Max. % Organic: 20 %
Shipping Solvent: water

P/N
Description
Particlesize
Housingmaterial ID(mm) Length(cm)
0008024
TSKgel G6000PWXL 13 µm Stainless Steel 7.8 30
0008033
Guard Column for P/N 0008024 12 µm Stainless Steel 6 4
0008035
TSKgel Top-Off for PWXL, 1g 10 µm


Base Material: hydroxylated polymethacylate
Particle Size (mean): 10 µm
Pore size (mean): > 100 nm
Working Range:  500 - 5,000 bp DNA
Calibration Range (polyethylene glycols and oxides):   40,000 - 8,000,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0008032
TSKgel G-DNA-PW
10 µm
Stainless Steel 7.8 30
0008033
Guard Column for P/N 0008032
12 µm
Stainless Steel 6 4
0008035
TSKgel Top-Off for P/N 0008032, 1g
10 µm
Base Material: hydroxylated polymethacylate
Particle Size (mean): 13 µm
Pore size (mean): 10 - 100 nm
Calibration Range (polyethylene glycols and oxides):   1,000 - 8,000,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0008025
TSKgel GMPWXL
13 µm
Stainless Steel 7.8 30
0008033
Guard Column for P/N 0008025
12 µm
Stainless Steel 6 4
0008035
TSKgel Top-Off for P/N 0008025, 1g
10 µm
Base Material: hydroxylated polymethacylate
Particle Size (mean): 7 µm
Pore size (mean): 12.5 nm
Calibration Range (polyethylene glycols and oxides):   up to 3,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0008031
TSKgel G-Oligo-PW
7 µm
Stainless Steel 7.8 30
0008034
Guard Column for P/N 0008031
13 µm
Stainless Steel 6 4
0008035
TSKgel Top-Off for P/N 0008031, 1g
10 µm