TSKgel SW column

TSKgel SW Columns for Protein Analysis

TSKgel SW columns, introduced in 1977, were the first of a long line of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.

TSKgel SW-type columns are based on highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SW-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes.

The calibration curves are shown for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SW-type columns. The table summarizes the molecular weight ranges for TSKgel G2000SW, G3000SW and G4000SW columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the particle pores. For comparative purposes, similar data is provided as well for TSKgel SWXL and SuperSW-type columns.

 

Literature :

 • Catalog

 • SEC Column Products Table

 • Instruction Manual

Base Material: Silica  
Particle Size (mean): 10 µm and 13 µm  
Pore size (mean): 12.5 nm  
Functional Group: Diol  
pH Stability: 2.5 - 7.5             
Calibration Range (globular proteins): 5,000 - 100,000 Da
 
Calibration Range (dextrans): 1,000 - 3 × 104   
Calibration Range (polyethylene glycols and oxides): 500 - 15,000
 

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0005788
TSKgel G2000SW
10μm
Stainless Steel 7.5 30
0005102 TSKgel G2000SW
10μm
Stainless Steel 7.5 60
0005371
Guard column for P/N 0005788 and 0005102
 
Stainless Steel 7.5 7.5
0006727
TSKgel G2000SW
13μm
Stainless Steel 21.5 30
0005146
TSKgel G2000SW
13μm
Stainless Steel 21.5 60
0005758
Guard Column for P/N 0006727 and 0005146
 
Stainless Steel 21.5 7.5
0006819
TSKgel SW Top-Off for 7.5mm ID SW, 1g
10μm
Base Material:  Silica
Particle Size (mean): 10 µm and 13 µm
Pore Size (mean): 25 nm
Functional Group: Diol
pH Stability: 2.5 - 7.5
Calibration Range (globular proteins): 10,000 - 500,000 
Calibration Range (dextrans):
2,000 - 7 x 104
Calibration Range (polyethylene glycols and oxides)
1,000 - 3.5 x 104

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0008800
TSKgel G3000SW
10μm
Glass 8 30
0008805
Guard Column for P/N 0008800
Glass 8 4
0005789
TSKgel G3000SW
10μm
Stainless Steel 7.5 30
0005103
TSKgel G3000SW
10μm
Stainless Steel 7.5 60
0005371
Guard Column for P/Ns 0005789, 0005103
 
Stainless Steel 7.5 7.5
0006819
TSKgel Top-Off for 7.5mmID SW, 1 g
10μm
Stainless Steel

0006728
TSKgel G3000SW
13μm
Stainless Steel 21.5 30
0005147
TSKgel G3000SW
13μm
Stainless Steel 21.5 60
0005758
Guard Column for P/Ns 0006728, 0005147
Stainless Steel 21.5 7.5 
Base Material:  Silica 
Particle Size (mean): 13 µm and 17 µm
Pore Size (mean): 45 nm
Functional Group: Diol
pH Stability: 2.5 - 7.5
Calibration Range (globular proteins): 20,000 - 7 × 106 
Calibration Range (dextrans): 4,000 - 5 x 105 
Calibration Range (polyethylene glycols and oxides):  
2,000 - 2.5 x 105

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
 0008801 TSKgel G4000SW
13μm
Glass 8 30
 0008805 Guard column for P/N 0008801
 
Glass 8 4
0005790
TSKgel G4000SW
13μm
Stainless Steel 7.5 30
0005104
TSKgel G4000SW
13μm
Stainless Steel 7.5 60
0005371
Guard column for P/N 0005790 and 0005104
 
Stainless Steel 7.5 7.5
0006819
TSKgel SW Top-Off, 1g for P/N 005790, 0005104
10μm
0006729
TSKgel G4000SW
17μm
Stainless Steel 21.5 30
0005148
TSKgel G4000SW
17μm
Stainless Steel 21.5 60
0005758
Guard column for P/N 0006729, 0005148
 
Stainless Steel 21.5 7.5