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TSKgel SWXL column

TSKgel SWXL Columns for Protein Analysis

TSKgel SWXL columns, introduced in 1987, represent the second generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.

Particle Characteristics

TSKgel SWXL-type columns contain smaller particles than TSKgel SW-type columns; 5 and 8 μm versus 10 and 13 μm. As the TSKgel SW-type columns, TSKgel SWXL columns feature highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SWXL-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes. These columns are commonly used in the quality control of monoclonal antibodies and other biopharma products.

Calibration Curves

The Figure shows the calibration curves for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SWXL-type columns. 

The tables below include the molecular weight ranges for TSKgel G2000SWXL, G3000SWXL and G4000SWXL columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the pores. 





Base Material: Silica 
Particle Size (mean): 5 µm
Pore Size (mean): 12.5 nm
Functional Group: Diol
pH Stability: 2.5 - 7.5
Calibration Range (globular proteins): 5,000-150,000
Calibration Range (dextrans): 1,000 - 3 x 104
Calibration Range (polyethylene glycols and oxides):   500 - 15,000

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0020027 TSKgel BioAssist G2SWXL
5μm
PEEK 7.8 30
0018008
Guard Column for P/N 0020027
7μm
PEEK 6 4
0008540
TSKgel G2000SWXL
5μm
Stainless Steel 7.8 30
0008543
Guard Column for P/N 0008540
7μm
Stainless Steel 6 4
0008544
TSKgel SWXL Top-Off for SWXL, 1g
5μm



Base Material:  Silica
Particle Size (mean): 5 μm
Pore Size (mean): 25 nm
Functional Group Diol
pH Stability 2.5 - 7.5
Calibration Range (globular proteins): 1 x 104 - 5 x 105 
Calibration Range (dextrans): 2,000 - 7 x 104
Calibration Range (polyethylene glycols and oxides):   1,000 - 3.5 x 104

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0020026 TSKgel BioAssist G3SWXL
5μm
PEEK 7.8 30
0018008
Guard Column for P/N 0020026
7μm
PEEK 6 4
0008541
TSKgel G3000SWXL
5μm
Stainless Steel 7.8 30
0008543
Guard Column for P/N 0008541
7μm
Stainless Steel 6 4
0008544
TSKgel SWXL Top-Off for SWXL, 1g
5μm



Base Material: Silica
Particle Size (mean): 8 µm
Pore Size (mean): 45 nm
Functional Group: Diol
pH Stability: 2.5 - 7.5
Calibration Range (globular proteins): 20,000 - 7 x 106 
Calibration Range (dextrans): 4,000 - 5 x 105
Calibration Range (polyethylene glycols and oxides):   2,000 - 2.5 x 105 

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0020025
TSKgel BioAssist G4SWXL
8μm
PEEK 7.8 30
0018008
Guard Column for P/N 0020025
 
PEEK
6
4
0008542
TSKgel G4000SWXL
8μm
Stainless Steel 7.8 30
0008543
Guard Column for P/N 0008542
7μm
Stainless Steel
6
4
0008544 TSKgel Top-Off for SWXL, 1g
5μm