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TSKgel SuperSW

TSKgel SuperSW Columns for Higher Sensitivity Protein Analysis


TSKgel SuperSW
columns, introduced in 1997, represent the third generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.

Particle Characteristic

TSKgel SuperSW-type columns contain smaller particles than TSKgel SWXL-type columns; 4 micron versus 5 micron. In addition, the column internal diameter has been reduced from 7.8 mm ID to 4.6 mm ID to provide higher sensitivity in sample-limited cases and to cut down on solvent use.

HPLC System Requirements

It is important to employ an HPLC system that is optimized with regards to extra-column bandbroadening to take full advantage of the high column efficiency that can be obtained on TSKgel SuperSW columns. See Separation Report 95 for recommendations.

Calibration Curves

The figure to the right shows the calibration curves for globular proteins for the two TSKgel SuperSW-type columns in comparison with those for the corresponding TSKgel SWXL columns. 

 
                              
Base Material:  Silica 
Particle Size (mean): 4 µm
Pore Size (mean): 12.5 nm
Functional Group: Diol
pH Stability: 2.5 - 7.5
Calibration Range (globular proteins): 5,000 - 150,000 
Calibration Range (dextrans): 1,000 - 3 x 104 
Calibration Range (polyethylene glycols and oxides):   500 - 15,000

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
 0018674 TSKgel SuperSW2000 4µm Stainless Steel 4.6 30
 0018762 Guard Column for P/N 0018674
 
Stainless Steel 4.6 3.5
Base Material:  Silica 
Particle Size (mean): 4 µm
Pore Size (mean): 25 nm
Functional Group: Diol
pH Stability: 2.5 - 7.5
Calibration Range (globular proteins): 1 x 104 - 5 x 105
Calibration Range (dextrans): 2,000 - 7 x 104
Calibration Range (polyethylene glycols and oxides):   1,000 - 3.5 x 104

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0018675
TSKgel SuperSW3000
4µm
Stainless Steel 4.6 30
0018762
Guard Column for P/N 0018675
Stainless Steel 4.6 3.5
0021485
TSKgel SuperSW3000
4µm
Stainless Steel 2 30
0021845
TSKgel SuperSW3000
4µm
Stainless Steel 1 30