TSKgel PW column

水溶性聚合物分析用TSKgel PW 色谱柱

TSKgel PW系列色谱柱由亲水性球形聚甲基丙烯酸脂颗粒填充,填料粒径覆盖从12µm(小孔径色谱柱)到17µm(大孔径色谱柱)。

TSKgel PW系列色谱柱的主要应用范围在于分析水溶性聚合物,如纤维素、丙烯酰胺、二醇、葡聚糖、聚乙烯醇和低聚糖等。


左上图为所有PW型柱的校准曲线。不同聚合物样品的适用溶剂请详见下表。

 
                                             
 

TSKgel PW型特制柱

TSKgel GMPW为混合型色谱柱,具有更宽范围的的线性校准范围,适合于MW分布较宽的样品和分子量未知的样品。 TSKgel G2000PW的粒径径较大(与TSKgel G-Oligo-PW相等),最适合低聚糖的半制备和制备分离。 TSKgel G1000PW是低分子量低聚物分析的首选。

聚合物类型

典型样品

适合的溶剂

非离子亲水型

聚乙二醇

可溶性淀粉、甲基纤维素 

右旋糖酐、羟乙基纤维素            

聚乙烯醇、聚丙烯酰胺

蒸馏水

0.01 mol/L NaOH          
           
20% DMSO
缓冲液或盐溶液( 例如:0.1-0.5 mol/L NaNO3)

            
 

非离子疏水型

 

聚乙烯吡咯烷酮

含有有机溶剂的缓冲液或盐溶液( 例如:0.1 mol/L NaNO3中添加20% CH3CN )

阴离子亲水性

硫酸软骨素钠、藻酸钠、羧甲基纤维素、聚丙烯酸钠、透明质酸钠

缓冲液或盐溶液 (例如:0.1 mol/L NaNO3)

阴离子疏水

磺化木质素钠盐、聚苯乙烯磺酸钠
             
            

含有有机溶剂的缓冲液或盐溶液( 例如:0.1 mol/L NaNO3中添加20% CH3CN)

阳离子亲水性

乙二醇壳聚糖、DEAE-葡聚糖、聚乙烯亚胺、聚三甲基氨基乙基甲基丙烯酸脂、碘化盐

含有0.3 mol/L Na2SO4 的0.5M乙酸或0.8 mol/L NaNO3

阳离子疏水

聚4-乙烯基苄基三甲基氯化铵、聚N-甲基-2-乙烯基吡啶、碘化盐

含有0.3 mol/L Na2SO4的0.5 mol/L 乙酸

两性亲水性

肽、蛋白质、多糖和寡糖、DNA、RNA

缓冲液和盐溶液( 例如:0.1 mol/L NaNO3)

两性疏水
 

蓝色葡聚糖、胶原、明胶、疏水性蛋白、疏水性肽

含有有机溶剂的缓冲液或盐溶液 (例如:0.1 mol/L NaNO3 中添加20% CH3CN或0.1% TFA中添加35-45% CH3CN)

Base Material: hydroxylated polymethacylate
Particle Size (mean): 12 µm
Pore size (mean): 12.5 nm
Calibration Range (polyethylene glycols and oxides):   up to 2,000 Da
Max. % Organic solvent: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0005761
TSKgel G2000PW
12 µm
Stainless Steel 7.5 30
0005105
TSKgel G2000PW
12 µm
Stainless Steel 7.5 60
0006763
Guard Column for P/N 0005761 and 0005105
12 µm
Stainless Steel 7.5 7.5
Base Material: hydroxylated polymethacrylate
Particle Size (mean): 12 µm and 17 µm
Pore size (mean): <20 nm
Calibration Range (polyethylene glycols and oxides):  
up to 3,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0008028
TSKgel G2500PW
12 µm
Stainless Steel 7.5 30
0008029
TSKgel G2500PW
12 µm
Stainless Steel 7.5 60
0006762
Guard Column for P/N 0008028, 0008029
12 µm
Stainless Steel 7.5 7.5
0016248
TSKgel G2500PW
17 µm
Stainless Steel 21.5 30
0008030
TSKgel G2500PW
17 µm
Stainless Steel 21.5 60
0006758
Guard Column for P/N 0016248, 0008030
17 µm
Stainless Steel 21.5 7.5
Base Material: hydroxylated polymethacylate
Particle Size (mean): 12 µm and 17 µm
Pore size (mean): 20 nm
Calibration Range (polyethylene glycols and oxides):   up to 50,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm)
Length (cm)
0005762
TSKgel G3000PW
12 µm
Stainless Steel 7.5
30
0005106
TSKgel G3000PW
12 µm
Stainless Steel 7.5
60
0006762
Guard Column for P/N 0005762, 0005106
12 µm
Stainless Steel 7.5
7.5
0016249
TSKgel G3000PW
17 µm
Stainless Steel 21.5
30
0005151
TSKgel G3000PW  17 µm
 Stainless Steel 21.5 60
0006758
Guard Column for P/N 0016249, 0005151
17 µm
Stainless Steel 21.5
7.5
Base Material: hydroxylated polymethacylate
Particle Size (mean): 17 µm
Pore size (mean): 50 nm
Calibration Range (polyethylene glycols and oxides):   up to 300,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm)
Length (cm)
0005763
TSKgel G4000PW
17 µm
Stainless Steel 7.5
30
0005107
TSKgel G4000PW
17 µm
Stainless Steel 7.5
60
0006762
Guard Column for P/N 0005763, 0005107
12 µm
Stainless Steel 7.5
7.5
0016250
TSKgel G4000PW
20 µm
Stainless Steel 21.5
30
0005152 TSKgel G4000PW 20 µm  Stainless Steel 21.5  60
0006758
Guard Column for P/N 0016250, 0005152
17 µm
Stainless Steel 21.5
7.5
Base Material: hydroxylated polymethacylate
Particle Size (mean): 17 µm
Pore size (mean): 100 nm
Calibration Range (polyethylene glycols and oxides):   up to 1,000,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0005764
TSKgel G5000PW
17 µm
Stainless Steel 7.5 30
0005108
TSKgel G5000PW
17 µm
Stainless Steel 7.5 60
0006762
Guard Column for P/N 0005764, 0005108
12 µm
Stainless Steel 7.5 7.5
0016251
TSKgel G5000PW
20 µm
Stainless Steel 21.5 30
0005153
TSKgel G5000PW
20 µm
Stainless Steel 21.5 60
0006758
Guard Column for P/N 0016251, 0005153 17 µm
Stainless Steel 21.5 7.5
Base Material: hydroxylated polymethacylate
Particle Size (mean): 17 µm
Pore size (mean): >100 nm
Calibration Range (polyethylene glycols and oxides):   up to 8,000,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0005765
TSKgel G6000PW
17 µm
Stainless Steel 7.5 30
0005109
TSKgel G6000PW
17 µm
Stainless Steel 7.5 60
0006762
Guard Column for P/N 0005765 and 0005109
12 µm
Stainless Steel 7.5 7.5
0020024
TSKgel BioAssist G6PW
17 µm
PEEK 7.8 30
Base Material: hydroxylated polymethacylate
Particle Size (mean): 17 µm
Pore size (mean): <10 - 100 nm
Calibration Range (polyethylene glycols and oxides):   500 - 8,000,000 Da
Max. % Organic: 20%
Shipping Solvent: water

P/N
Description
Particlesize
Housingmaterial ID(mm) Length(cm)
0008026
TSKgel GMPW
17 µm
Stainless Steel 7.5 30
0008027
TSKgel GMPW
17 µm
Stainless Steel 7.5 60
0006762
Guard Column for P/N 0008026 and 0008027
12 µm
Stainless Steel 7.5 7.5