TOYOPEARL HW-40 resin is a hydroxylated methacrylic polymer for size exclusion chromatography. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.
TOYOPEARL HW-40 resin is available in three particle sizes.
| Pore size (mean) | 5 nm
|
| Exclusion limit | 10,000 Da |
| Particle size (mean) | 75 µm (C-grade)
45 µm (F-grade)
30 µm (S-grade) |
| Pressure rating | 0.3 MPa |
| Shipping buffer | 20% ethanol |
| pH stability | 2 - 13 |
| P/N | Description | Resin volume | Particle size |
| 0007450 | Toyopearl HW-40EC | 500 mL | 100 - 300 µm |
| 0007449 | Toyopearl HW-40C | 500 mL | 50 - 100 µm |
| 0014683 | Toyopearl HW-40C | 1 L | 50 - 100 µm |
| 0007969 | Toyopearl HW-40C | 5 L | 50 - 100 µm |
| 0007448 | Toyopearl HW-40F | 500 mL | 30 - 60 µm |
| 0014682 | Toyopearl HW-40F | 1 L | 30 - 60 µm |
| 0007968 | Toyopearl HW-40F | 5 L | 30 - 60 µm |
| 0007447 | Toyopearl HW-40S | 500 mL | 20 - 40 µm |
| 0014681 | Toyopearl HW-40S | 1 L | 20 - 40 µm |
| 0007967 | Toyopearl HW-40S | 5 L | 20 - 40 µm |
TOYOPEARL HW-50 resin is a hydroxylated methacrylic polymer for size exclusion chromatography of proteins between 500-80,000 Da.
TOYOPEARL HW-50 resin is available in two particle sizes.
| Pore size (mean) | 12.5 nm |
| Exclusion limit | 80,000 Da |
| Particle size (mean) | 45 µm (F-grade)
30 µm (S-grade) |
| Pressure rating | 0.3 MPa |
| Shipping buffer | 20% ethanol |
| pH stability | 2 - 13 |
| P/N | Description | Resin volume | Particle size |
| 0007453 | Toyopearl HW-50F | 500 mL | 30 - 60 µm |
| 0014685 | Toyopearl HW-50F | 1 L | 30 - 60 µm |
| 0008060 | Toyopearl HW-50F | 5 L | 30 - 60 µm |
| 0007452 | Toyopearl HW-50S | 500 mL | 20 - 40 µm |
| 0014684 | Toyopearl HW-50S | 1 L | 20 - 40 µm |
| 0008059 | Toyopearl HW-50S | 5 L | 20 - 40 µm |
TOYOPEARL HW-55 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 1,000-700,000 Da. It also is the base bead for all TOYOPEARL "-550" designated products.
TOYOPEARL HW-55 resin is available in two particle sizes.
| Pore size (mean) | 50 nm |
| Exclusion limit | 700,000 Da |
| Particle size (mean) | 45 µm (F-grade)
30 µm (S-grade) |
| Pressure rating | 0.3 MPa |
| Shipping buffer | 20% ethanol |
| pH stability | 2 - 13 |
| P/N | Description | Resin volume | Particle size |
| 0007457 | Toyopearl HW-55F | 500 mL | 30 - 60 µm |
| 0014687 | Toyopearl HW-55F | 1 L | 30 - 60 µm |
| 0008063 | Toyopearl HW-55F | 5 L | 30 - 60 µm |
| 0007456 | Toyopearl HW-55S | 500 mL | 20 - 40 µm |
| 0014686 | Toyopearl HW-55S | 1 L | 20 - 40 µm |
| 0008062 | Toyopearl HW-55S | 5 L | 20 - 40 µm |
TOYOPEARL HW-65 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 40,000-5,000,000 Da. It also is the base bead for all TOYOPEARL "-650" designated products.
TOYOPEARL HW-65 SEC resin is available in two particle sizes.
| Pore size (mean) | 100 nm |
| Exclusion limit | 5,000,000 Da |
| Particle size (mean) | 45 µm (F-grade)
30 µm (S-grade) |
| Pressure rating | 0.3 MPa |
| Shipping buffer | 20% ethanol |
| pH stability | 2 - 13 |
| P/N | Description | Resin volume | Particle size |
| 0007465 | Toyopearl HW-65F | 500 mL | 30 - 60 µm |
| 0014689 | Toyopearl HW-65F | 1 L | 30 - 60 µm |
| 0008069 | Toyopearl HW-65F | 5 L | 30 - 60 µm |
| 0007464 | Toyopearl HW-65S | 500 mL | 20 - 40 µm |
| 0014688 | Toyopearl HW-65S | 1 L | 20 - 40 µm |
| 0008068 | Toyopearl HW-65S | 5 L | 20 - 40 µm |
TOYOPEARL HW-75 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 500,000-50,000,000 Da.
TOYOPEARL HW-75 resin is available in two particle sizes.
| Pore size (mean) | > 100 nm |
| Exclusion limit | 50,000,000 Da |
| Particle size (mean) | 45 µm (F-grade)
30 µm (S-grade) |
| Pressure rating | 0.3 MPa |
| Shipping buffer | 20% ethanol |
| pH stability | 2 - 13 |
| P/N | Description | Resin volume | Particle size |
| 0007469 | Toyopearl HW-75F | 500 mL | 30 - 60 µm |
| 0014691 | Toyopearl HW-75F | 1 L | 30 - 60 µm |
| 0008072 | Toyopearl HW-75F | 5 L | 30 - 60 µm |
| 0007468 | Toyopearl HW-75S | 500 mL | 20 - 40 µm |
| 0008071 | Toyopearl HW-75S | 5 L | 20 - 40 µm
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