TOYOPEARL AF-rProtein A-650


TOYOPEARL AF-rProtein A-650F utilizes an alkaline resistant recombinant Protein A variant. This new, non-animal derived, ligand is bound to the base bead, TOYOPEARL HW-65F, using multi-point attachment. This multi-point attachment contributes to both this resin's alkaline resistance and its low ligand leakage (approx. 5-25 ng/mg IgG).

TOYOPEARL AF-rProtein A-650F maintains its capacity after repeated CIP exposure to 0.1 mol/L NaOH and has reasonable tolerance to 0.5 mol/L NaOH CIP.

This Protein A resin also tolerates exposure to temperatures above ambient and if needed can be autoclaved.

Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at low residence times.

CIP Stability (0.5 mol/L NaOH)

DBC at Various Residence Times

CIP Stability (0.1 mol/L NaOH)

Temperature Stability

Literature :

 • Catalog

 • Instruction Manual