TOYOPEARL AF-rProtein A HC-650


TOYOPEARL AF-rProtein A HC-650F resin is the high capacity protein A resin. An enhanced rProtein A ligand is bound to the TOYOPEARL HW-65F base bead via multipoint attachment resulting in excellent base stability for up to 200 CIP cycle with 0.1 mol/L NaOH. TOYOPEARL AF-rProtein A HC-650F resin maintains 80% of initial dynamic binding capacity after 40 CIP cycles with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A HC-650F resin exhibits dynamic binding capacities of greater than 65 g/L at residence times of 5 minutes and greater than 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L.
Achievement of high linear velocities at relatively low pressure enables high throughput at production scale using equipment with moderate pressure limitations. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to agarose base stable resins.


 CIP Stability (0.1 mol/L NaOH)

 DBC at Various Residence Times

DBC at Various Residence Times


 CIP Stability (0.5 mol/L NaOH)

Literature :

 • Catalog

 • Instruction Manual