Anion Exchange Chromatography

Anion Exchange Chromatography

Anion exchange chromatography is practiced with either a strong or a weak anion exchange column, containing a quarternary ammonium ion, or with a weak anion exchanger, having either a tertiary or secondairy amine functional group, such as DEAE (diethylaminoethyl). A counter ion, often Cl- maintains electroneutrality.

In ion exchange chromatography, the pH of the mobile phase buffer must be between the pI or pKa of the charged molecule and the pKa of the charged groups on the solid support. For instance, in anion exchange chromatography a molecule with a pI of 6.8 is run in a mobile phase buffer at pH 8.0 with the solid support pKa at 10.3.


Highlights:

  • Pore structure and bonding chemistry of TSKgel BioAssist Q columns provides high capacity for small to very large molecular weight proteins and nucleic acids
  • TSKgel SuperQ-5PW columns have higher capacity than TSKgel DEAE-5PW due to novel bonding chemistry; effective pore size is smaller for TSKgel SuperQ-5PW
  • Binding capacity for small to medium size proteins on TSKgel DEAE-3SW is roughly double that of the 5PW packings due to the smaller pore size and larger surface area.
  • TSKgel DEAE-5PW and DEAE-2SW columns are available in 2 mm ID format
  • Specialty columns for analysis of mono- and disaccharides, sugar alcohols, and organic acids.
  • TSKgel DNA-NPR columns for PCR fragment analysis
  • TSKgel BioAssist columns are packed in PEEK hardware, while other columns are available in glass and stainless steel.
  • Very efficient chromatography can be achieved with the TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores. 

Speciality Columns

Oligonucleotide fragments, PCR reagents (TSKgel DNA-NPR)
Mono- and disaccharides (TSKgel Sugar AXI and TSKgel Sugar AXG)


Literature:

 • Separation Report No. 100

 • Catalog

 • IEC Column Products Table


Base Material:
polymethacylate
Particle Size (mean):  
10 µm and 13 µm
Pore Size (mean): 400 nm
Functional Group: polyamine
Counter Ion: Cl-
pH Range: 2.0 - 12.0
Protein Capacity  : 70 g BSA/L
 
Small Ion Capacity: 0.1 eq/L  
pKa: 9.4  

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0019685
TSKgel BioAssist Q
10 µm
PEEK 4.6 5
0021410
TSKgel BioAssist Q
13 µm
PEEK 10 10

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: hydrophilic silica
Particle Size (mean):   5 µm
Pore Size (mean): 12.5 nm
Functional Group: CH2CH2N+(C2H5)3
Counter Ion: H2PO4-
pH Range: 2.0 - 7.5
Protein Capacity : ND  
Small Ion Capacity: > 0.3 meq/g
pKa: 11.2

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0018761
TSKgel DEAE-2SW
5 µm
Stainless Steel 2 25
0007168
TSKgel DEAE-2SW
5 µm
Stainless Steel 4.6 25
0007648
Guardgel Kit for P/N 0007168

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: hydrophilic silica
Particle Size (mean):   10 µm
Pore Size (mean): 25 nm
Functional Group: CH2CH2N+(C2H5)3
Counter Ion: Cl-
pH Range: 2.0 - 7.5
Protein Capacity
ND  
Small Ion Capacity: > 0.3 eq/g  
pKa: 11.2  


P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0007163
TSKgel DEAE-3SW
10 µm
Stainless Steel 7.5 7.5
0007648
Guardgel Kit for P/N 0007163
 
Stainless Steel

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual

 • Instruction Manual for Glass Columns


Base Material: hydroxylated methacrylate polymer
Particle Size (mean):   10, 13 and 20 µm
Pore Size (mean): 100 nm
Functional Group: CH2CH2N+(C2H5)3
Counter Ion: Cl-
pH Range: 2.0 - 12.0
Protein Capacity : 30 g BSA/L
 
Small Ion Capacity: 0.1 eq/L  
pKa: 11.5  

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0013061
TSKgel DEAE-5PW
10 µm
Glass 5 5
0008802
TSKgel DEAE-5PW
10 µm
Glass 8 7.5
0008806
Glass Guardgel Kit for P/N 0013061, 0008802
 
Glass

0014016
TSKgel DEAE-5PW
13 µm
Glass 20 15
0014466
Glass Guard Column for P/N 0014016
 
Glass 20
2
0018757
TSKgel DEAE-5PW
10 µm
Stainless Steel 7.5 
0007164
TSKgel DEAE-5PW                        
10 µm
Stainless Steel 7.5 7.5
0007210
Guardgel Kit for P/N 0007164
 
Stainless Steel

0007574
TSKgel DEAE-5PW  13 µm Stainless Steel 21.5 15
0016092
Guardgel Kit for P/N 0007574   Stainless Steel

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: hydroxylated methacrylate polymer
Particle Size (mean): 2.5 µm
Pore Size (mean):   non-porous
Functional Group: CH2CH2N+(C2H5)3
Counter Ion: Cl-
pH Range: 2.0 - 12.0
Protein Capacity : 5 g BSA/L  
Small Ion Capacity: > 0.1 eq/L  
pKa: 11.2  

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0013075
TSKgel DEAE-NPR
2.5 µm
Stainless Steel 4.6 3.5
0017088
Guard Column for P/N 0013075
5 µm
Stainless Steel 4.6 0.5

Literature:

 • Catalog

 • IEC Column Products Table


Base Material: hydroxylated methacrylate polymer
Particle Size (mean):   2.5 µm
Pore Size (mean): non-porous
Functional Group: proprietary
Counter Ion: ClO4-
pH Range: 2.0 - 12.0
Protein Capacity : 5 g BSA/L
 
Small Ion Capacity: 0.1 eq/L  
pKa: 11.2  

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0018249
TSKgel DNA-NPR
2.5 µm
Stainless Steel 4.6 7.5
0018253
Guard Column  for P/N 0018249  5 µm
Stainless Steel 4.6 0.5

Literature:

 • Separation Report No. 109

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: hydrophilic polymer
Particle Size (mean):   5 µm
Pore Size (mean): non-porous
Functional Group: quarternary ammonium
Counter Ion: Cl-
pH Range: 3.0 - 10.0
Static Binding Capacity (mg BSA/g dry gel):   ca. 35 (5 µm)
 
Small Ion Capacity: 270 µeg/g dry gel
 
pKa: 10.5
 
Hardware: Stainless Steel tubing & fittings, PEEK frits

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0021962
TSKgel DNA-STAT
5 µm
Stainless Steel 4.6 10

Literature:

 • Catalog

 • IEC Column Products Table


Base Material: silica
Particle Size (mean):   5 µm
Pore Size (mean): 12.5 nm
Functional Group: trimethylamino
Counter Ion: H2PO4-
pH Range: 2.0 - 7.5
Protein Capacity : ND  
Small Ion Capacity: > 0.3 meq/g  
pKa: 11.2  

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0007166
TSKgel QAE-2SW
5 µm
Stainless Steel 4.6 25
0007646
Guardgel Kit for P/N 0007166
 
Stainless Steel

Literature:

 • Separation Report No. 109

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: hydrophilic polymer
Particle Size (mean): 7 µm and 10 µm
Pore Size (mean): non-porous
Functional Group: quarternary ammonium
Counter Ion: Cl-
pH Range: 3.0 - 10.0
Static Binding Capacity  
(mg BSA/g dry gel):
ca. 25 (7 µm)
ca. 20 (10 µm)
 
Small Ion Capacity: 270 µeg/g dry gel
 
pKa: 10.5  
Hardware: Stainless Steel tubing & fittings, PEEK frits  

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0021960
TSKgel Q-STAT
10 µm
Stainless Steel 3 3.5
0021961
TSKgel Q-STAT
7 µm
Stainless Steel 4.6 10

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: PS-DVB polymer
Particle Size (mean):   5 µm
Pore Size (mean): 6.0 nm
Functional Group: trimethylamine
Counter Ion: Cl-
pH Range: 1.0 - 14.0
Protein Capacity
ND  
Small Ion Capacity: 1.5 eq/L  
pKa: 12.5  

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0007157
TSKgel SAX
5 µm
Stainless Steel 6 15

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: PS-DVB polymer
Particle Size (mean):   10 µm
Pore Size (mean): 6.0 nm
Functional Group: trimethylamino
Counter Ion: HBO32-
pH Range: 1.0 - 14.0
Protein Capacity : NA  
Small Ion Capacity: > 1.2 eq/L  
pKa: 12.5

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0008640
TSKgel Sugar AXG
10 µm
Stainless Steel 4.6 15

Literature:

 • Catalog

 • IEC Column Products Table

 • Instruction Manual


Base Material: PS-DVB polymer
Particle Size (mean):   8 µm
Pore Size (mean): 6.0 nm
Functional Group: trimethylamino
Counter Ion: HBO32-
pH Range: 1.0 - 14.0
Protein Capacity : ND  
Small Ion Capacity: > 1.2 eq/L  
pKa: 12.5  

P/N
Description
Particle size
Housing material ID (mm) Length (cm)
0008639
TSKgel Sugar AXI
8 µm
Stainless Steel 4.6 15

Literature:

 • Catalog

 • IEC Column Products Table


Base Material: polymethacrylate
Particle Size (mean):   10 µm and 13 µm
Pore Size (mean): 100 nm
Functional Group: trimethylamino
Counter Ion: Cl-
pH Range: 2.0 - 12.0
Protein Capacity : 100 g BSA/L
 
Small Ion Capacity: > 0.13 eq/L  
pKa: 12.2  

P/N
Description
Particle size
Housing material ID (mm)
Length (cm)
0018386
TSKgel SuperQ-5PW
10 µm
Glass 8
7.5
 0018389 Guardgel Kit for P/N 0018386   Glass    
0018257
TSKgel SuperQ-5PW
10 µm
Stainless Steel 7.5
7.5
0018388
Guardgel Kit for P/N 0018257
Stainless Steel  
0018387
TSKgel SuperQ-5PW
13 µm
Stainless Steel 21.5
15 
 0018390 Guardgel Kit for P/N 0018387
   Stainless Steel