TSKgel PWXL | https://separations.asia.tosohbioscience.com

TSKgel PWXL Columns for Analysis of Water-Soluble Polymers

TSKgel PWXL columns are composed of spherical, hydrophilic polymethacrylate beads. The main application area for TSKgel PWXL columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. See the table on the TSKgel PW page for typical samples and suitable eluents.

Figure 1 shows the calibration curves for all TSKgel PWXL-type columns. Figure 2 shows the benefits of TSKgel PWXL columns over TSKgel PW columns, namely the improved resolution due to the smaller particle sizes used in TSKgel PWXL columns.

Figure 1: Polyethylene glycol and oxide

calibration curves on TSKgel PWXL columns

Figure 2: Faster analysis and higher resolution with TSKgel PWXL columns.

Although proteins can be analyzed on TSKgel PWXL columns, pore volumes are much smaller than on TSKgel SW-type columns. Figure 3 demonstrates the limited separation power of TSKgel PWXL for protein samples.

Specialty Columns

TSKgel G-DNA-PW is available for the analysis of DNA fragments. Similarly, TSKgel G-Oligo-PW is the preferred column for the analysis of oligosaccharides.

The mixed-mode column, TSKgel GMPWXL, has an extended linear calibration range, suitable for samples with a broad MW distribution and unknowns.

A smaller particle size version of the popular TSKgel G-Oligo-PW column for oligomer separation is now also available. By using 3 micron instead of 7 micron particles a separation performed on an TSKgel G-Oligo-PW column can be done in half the time on a TSKgel SuperOligoPW column.

Figure 3: Protein Calibration curves for TSKgel PWXL

Literature :

• Separation Report No.37

• Separation Report No.38

• Catalog

• SEC Column Products Table

• Instruction Manual

Base Material:	hydroxylated polymethacylate
Particle Size (mean):	7 µm
Pore size (mean):	< 20 nm
Calibration Range (polyethylene glycols and oxides):	< 3,000 Da
Max. % Organic:	20%
Shipping Solvent:	water

P/N	Description	Particle size	Housingmaterial	ID (mm)	Length (cm)
0008020	TSKgel G2500PW_XL	7 µm	Stainless Steel	7.8	30
0008033	Guard Column for P/N 0008020	12 µm	Stainless Steel	6	4
0008035	TSKgel Top-Off for PW_XL, 1g	10 µm

Base Material:	hydroxylated polymethacylate
Particle Size (mean):	7 µm
Pore size (mean):	20 nm
Calibration Range (polyethylene glycols and oxides):	< 40,000 Da
Max. % Organic:	20%
Shipping Solvent:	water

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0008021	TSKgel G3000PW_XL	7 µm	Stainless Steel	7.8	30
0008033	Guard Column for P/N 0008021	12 µm	Stainless Steel	6	4
0008035	TSKgel Top-Off for PW_XL, 1g	10 µm

Base Material:	hydroxylated polymethacylate
Particle Size (mean):	10 µm
Pore size (mean):	< 50 nm
Calibration Range (polyethylene glycols and oxides):	2,000 - 300,000 Da
Max. % Organic:	20%
Shipping Solvent:	water

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0008022	TSKgel G4000PW_XL	10 µm	Stainless Steel	7.8	30
0008033	Guard Column for P/N 0008022	12 µm	Stainless Steel	6	4
0008035	TSKgel Top-Off for PW_XL, 1g	10 µm

Base Material:	hydroxylated polymethacylate
Particle Size (mean):	10 µm
Pore size (mean):	100 nm
Calibration Range (polyethylene glycols and oxides):	4,000 - 800,000 Da
Max. % Organic:	20%
Shipping Solvent:	water

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0008023	TSKgel G5000PW_XL	10 µm	Stainless Steel	7.8	30
0008033	Guard Column for P/N 0008023	12 µm	Stainless Steel	6	4
0008035	TSKgel Top-Off for PW_XL, 1g	10 µm

Base Material:	hydroxylated polymethacylate
Particle Size (mean):	13 µm
Pore size (mean):	> 100 nm
Calibration Range (polyethylene glycols and oxides):	40,000 - 8,000,000 Da
Max. % Organic:	20 %
Shipping Solvent:	water

P/N	Description	Particlesize	Housingmaterial	ID(mm)	Length(cm)
0008024	TSKgel G6000PW_XL	13 µm	Stainless Steel	7.8	30
0008033	Guard Column for P/N 0008024	12 µm	Stainless Steel	6	4
0008035	TSKgel Top-Off for PW_XL, 1g	10 µm

Base Material:	hydroxylated polymethacylate
Particle Size (mean):	10 µm
Pore size (mean):	> 100 nm
Working Range:	500 - 5,000 bp DNA
Calibration Range (polyethylene glycols and oxides):	40,000 - 8,000,000 Da
Max. % Organic:	20%
Shipping Solvent:	water

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0008032	TSKgel G-DNA-PW	10 µm	Stainless Steel	7.8	30
0008033	Guard Column for P/N 0008032	12 µm	Stainless Steel	6	4
0008035	TSKgel Top-Off for P/N 0008032, 1g	10 µm

Base Material:	hydroxylated polymethacylate
Particle Size (mean):	13 µm
Pore size (mean):	10 - 100 nm
Calibration Range (polyethylene glycols and oxides):	1,000 - 8,000,000 Da
Max. % Organic:	20%
Shipping Solvent:	water

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0008025	TSKgel GMPW_XL	13 µm	Stainless Steel	7.8	30
0008033	Guard Column for P/N 0008025	12 µm	Stainless Steel	6	4
0008035	TSKgel Top-Off for P/N 0008025, 1g	10 µm

Base Material:	hydroxylated polymethacylate
Particle Size (mean):	7 µm
Pore size (mean):	12.5 nm
Calibration Range (polyethylene glycols and oxides):	up to 3,000 Da
Max. % Organic:	20%
Shipping Solvent:	water

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0008031	TSKgel G-Oligo-PW	7 µm	Stainless Steel	7.8	30
0008034	Guard Column for P/N 0008031	13 µm	Stainless Steel	6	4
0008035	TSKgel Top-Off for P/N 0008031, 1g	10 µm