TSKgel PWXL Columns for Analysis of Water-Soluble Polymers
TSKgel PW
XL columns are composed of spherical, hydrophilic polymethacrylate beads. The main application area for TSKgel PW
XL columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. See the table on the TSKgel PW page for typical samples and suitable eluents.
Figure 1 shows the calibration curves for all TSKgel PW
XL-type columns. Figure 2 shows the benefits of TSKgel PW
XL columns over TSKgel PW columns, namely the improved resolution due to the smaller particle sizes used in TSKgel PW
XL columns
.
Figure 1: Polyethylene glycol and oxide
calibration curves on TSKgel PWXL columns
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Figure 2: Faster analysis and higher resolution with TSKgel PWXL columns.
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![](/Image Library/TBJS/img/Columns/SEC/SEC_PWxl_PEG_PEOCurves.jpg?code=fe64be01-85a5-47a4-86d1-cb3aa61add13) |
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![](http://prod.separations.us.tosohbioscience.com/Image%20Library/TBL/HPLC%20Images/Images%20to%20Replace/SEC/SEC_PWxl_fig2.jpg?code=fbe43331-090f-482a-9614-0f3085453da6) |
Although proteins can be analyzed on TSKgel PWXL columns, pore volumes are much smaller than on TSKgel SW-type columns. Figure 3 demonstrates the limited separation power of TSKgel PWXL for protein samples.
Specialty Columns
TSKgel G-DNA-PW is available for the analysis of DNA fragments. Similarly, TSKgel G-Oligo-PW is the preferred column for the analysis of oligosaccharides.
The mixed-mode column, TSKgel GMPWXL, has an extended linear calibration range, suitable for samples with a broad MW distribution and unknowns.
A smaller particle size version of the popular TSKgel G-Oligo-PW column for oligomer separation is now also available. By using 3 micron instead of 7 micron particles a separation performed on an TSKgel G-Oligo-PW column can be done in half the time on a TSKgel SuperOligoPW column.
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Figure 3: Protein Calibration curves for TSKgel PWXL
![](/Image Library/TBJS/img/Columns/SEC/SEC_PWxl_ProteinCurves.jpg?code=9edab22b-ed20-445b-b6d7-c5252e4d917f) |