TSKgel SuperHZ Columns for Reduced Polymer Adsorption and Limited Solvent Range
The TSKgel SuperHZ column line consists of five columns with different pore sizes, TSKgel SuperHZ1000 through TSKgel SuperHZ4000, and three columns with an extended linear range of the calibration curve, TSKgel SuperHZM-N, SuperHZM-M and SuperHZM-H. The calibration curves are shown below.
Main characteristics:
- TSKgel SuperHZ columns have been developed for High Throughput, High Efficiency GPC applications such as those encountered in combinatorial chemistry experiments.
- The benefits of TSKgel SuperHZ columns over conventional TSKgel HXL columns are shown in the following chromatogram.
- Ultra-low sample adsorption, i.e., the columns show true size exclusion behavior for most polymers
- Maximum operating temperature for TSKgel SuperHZ-type columns is 60 ºC for TSKgel SuperHZ1000, SuperHZ2000, SuperHZ2500 and SuperHZ3000, and 80 ºC for the remaining columns in the product line. See TSKgel SuperH-type columns for operating temperatures of 140 ºC.
- Limited solvent range. The columns are shipped in THF, which can be exchanged for a limited number of organic solvents as shown below.
Calibration Curves for TSKgel SuperHZ Columns
Solvent compatibility for TSKgel SuperHZ columns
The column shipping solvent limits the types of solvents that can be used with each column type.
Recommended Solvent
|
Application
|
| THF |
polystyrene, epoxy resin, phenoxy resin, polycarbonate,
polyisoprene, polyvinyl acetate, polyvinyl chloride,
monoglycerides, fatty acids, poly(butadiene),
polybutadiene, poly(methyl methacrylate),
poly(styrene-butadiene), poly(styrene-acrylonitrile) |
N,N-Dimethylformamide (DMF) +/- 5mM LiBr
|
polyvinyl chloride, polyvinyl fluoride, urea resins,
polyurethane, polystyrene, polyester,
polyimido ether, polyimido ester,
polyphenol (aqueous solution), polyacrylonitrile
|
o-Dichlorobenzene (ODCB)
|
polyethylene, polypropylene
|
| Chloroform |
polycarboxylic ether, acrylic resin,
epoxy resin, polystyrene |
| m-Cresol/Chloroform |
nylon, polyester, polyamide,
poly(ethylene terephthalate) |
| Toluene |
polybutadiene, polysiloxane
|
Beware of extra-column bandbroadening
Column dimensions are 6 mm ID x 15 cm and 4.6 mm ID x 15 cm versus 7.8 mm ID x 30 cm for conventional GPC columns. The smaller column dimensions translate to a reduction of peak volume by a factor of 3.4 (6 mm ID) and a factor of 5.8 (4.6 mm ID) versus the same component eluting from a corresponding TSKgel HXL column. Thus, your HPLC system may require optimization of components that can give rise to extra-column bandbroadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.
Columns with linear calibration curves
The TSKgel SuperHZ product line contains three linear, or mixed-bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The mixed-bed columns feature increasing linear calibration ranges, from TSKgel SuperHZM-L, SuperHZM-N, SuperHZM-H.
Shipping Solvent
|
Can Be Replaced With
|
Tetrahydrofuran
|
benzene, chloroform, toluene, xylene,
dichloromethane, dichloroethane
|
| Acetone** |
carbon tetrachloride4, o-dichlorobenzene, dimethylformamide,
dodecane, dimethyl sulfoxide, dioxane, ethylacetate,
FC-113, hexane, pyridine, hexafluoroisopropanol/chloroform,
methyl ethyl ketone, quinoline, cyclohexane
|
| Chloroform** |
m-cresol in chloroform, up to 10%
hexafluoroisopropanol in chloroform
|
| Dimethylformamide |
dimethyl sulfoxide, dioxane, tetrahydrofuran, toluene |
| o-Dichlorobenzene |
1-chloronaphthalene, trichlorobenzene
|
Notes:
- In case of TSKgel SuperHZ and HXL, keep flow rate below <0.5 mL/min during solvent change. Solvent can be changed one way/one time only.
- All TSKgel HXL, HHR, SuperHZ and SuperH analytical columns are shipped containing tetrahydrofuran (THF), except High Temperature (HT) columns, which contain o-dichlorobenzene.
- Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.
* 100% methanol cannot be used with TSKgel H-type columns; use this solvent with TSKgel SW-type or Alpha-type columns.
** TSKgel H columns may be specially ordered with this shipping solvent.