TSKgel SW mAb Columns
The TSKgel SW mAb products consist of two specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAb can be analyzed using many different modes of HPLC, size exclusion is best for aggregation, dimer, and fragmentation, making it the best method for heterogeneity studies. The TSKgel SW mAb columns are:
- TSKgel SuperSW mAb HR for high resolution analysis of mAb monomer and dimer
- TSKgel UltraSW Aggregate for the analysis of mAb aggregates
Compared to competitive columns, these stainless steel, silica-based TSKgel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates.
The TSKgel SW mAb columns utilize a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody. As shown in the figure, the calibration curve for the TSKgel SuperSW mAb HR column is similar to that of the TSKgel G3000SWXL column curve and has a shallower slope than the TSKgel UltraSW Aggregate column around the molecular weight range of gamma-globulin. This shallow calibration curve produces high resolution separations. The TSKgel UltraSW Aggregrate calibration curve shows a separation range up to around 2 million Da, which implies better resolution of aggregate/multimer of a mAb.
Protein Calibration Curves for TSKgel SW mAb Columns
