Cation Exchange | https://separations.asia.tosohbioscience.com

Cation Exchange Chromatography

Cation exchange chromatography is practiced with either a strong or a weak cation exchange column, containing a sulfonium ion, or with a weak cation exchanger, having usually a carboxymethyl (CM) functional group. A counter ion, often Na+ maintains electroneutrality.

In ion exchange chromatography the pH of the mobile phase buffer must be between the pI or pKa of the charged molecule and the pKa of the charged groups on the solid support. For example, a molecule with a pI of 8.2 is run in a mobile phase buffer at pH 6.0 with the solid support pKa at 1.2 in cation exchange chromatography.

Highlights:

Pore structure and bonding chemistry of TSKgel BioAssist S provides high capacity for medium to large molecular weight proteins.
TSKgel BioAssist columns are packed in 4.6 mm ID or 10 mm ID PEEK hardware. Other columns are available in glass and stainless steel for analytic, semi-preparative and preparative applications.
Binding capacity for small to medium size proteins on TSKgel DEAE-3SW is approximately double that of the 5PW packings due to the smaller pore size and larger surface area.
TSKgel SP-5PW column available in 2 mm ID format.
Very efficient chromatography can be achieved with the TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores.

Specialty columns:

Organic acids by ion-exclusion (TSKgel OApak-A and TSKgel SCX)

Literature:

• Separation Report No. 100

• Catalog

• IEC Column Products Table

Base Material:	polymethacrylate
Particle Size (mean):	7 µm and 13 µm
Pore Size (mean):	130 nm
Functional Group:	sulfopropyl
Counter Ion:	Na⁺
pH Range:	2.0 - 12.0
Protein Capacity :	70 g gamme globulin/L
Small Ion Capacity:	0.1 eq/L
pKa:	2.4

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0019686	TSKgel BioAssist S	7 µm	PEEK	4.6	5
0021411	TSKgel BioAssist S	13 µm	PEEK	10	10

Literature:

• Catalog

• IEC Column Products Table

• Instruction Manual

Base Material:	spherical silica
Particle Size (mean):	5 µm
Pore Size (mean):	12.5 nm
Functional Group:	-CH₂COO^-
Counter Ion:	Na⁺
pH Range:	2.0 - 7.5
Protein Capacity :	ND
Small Ion Capacity:	> 0.3 meq/g
pKa:	4.2

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0007167	TSKgel CM-2SW	5 µm	Stainless Steel	4.6	25
0007650	Guardgel Kit for P/N 0007167

Literature:

• Catalog

• IEC Column Products Table

• Instruction Manual

Base Material:	spherical silica
Particle Size (mean):	10 µm
Pore Size (mean):	25 nm
Functional Group:	-CH₂COO^-
Counter Ion:	Na⁺
pH Range:	2.0 - 7.5
Protrein Capacity :	ND
Small Ion Capacity:	> 0.3 meq/g
pKa:	4.2

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0007162	TSKgel CM-3SW	10 µm	Stainless Steel	7.5	7.5
0007650	Guardgel Kit for P/N 0007162

Literature:

• Catalog

• IEC Column Products Table

• Instruction Manual

• Instruction Manual for Glass Columns

Base Material:	hydroxylated methacrylic polymer
Particle Size (mean):	10 µm and 13 µm
Pore Size (mean):	100 nm
Functional Group:	-CH₂COO^-
Counter Ion:	Na⁺
pH Range:	2.0 - 12.0
Protein Capacity :	45 mg Hb/mL
Small Ion Capacity:	> 0.1 eq/L
pKa:	4.2

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0013068	TSKgel CM-5PW	10 µm	Stainless Steel	7.5	7.5
0013069	Guardgel Kit for P/N 0013068		Stainless Steel
0014021	TSKgel CM-5PW	13 µm	Stainless Steel	21.5	15
0016094	Guardgel Kit for P/N 0014021		Stainless Steel
0014010	TSKgel CM-5PW	10 µm	Glass	5	5
0014011	TSKgel CM-5PW	10 µm	Glass	8	7.5
0014024	Guardgel Kit for P/N 0014011		Glass
0014012	TSKgel CM-5PW	13 µm	Glass	20	15
0014468	Guard Column for P/N 0014012		Glass	20	2

Literature:

• Separation Report No. 109

• Catalog

• IEC Column Products Table

• Instruction Manual

Base Material:	hydrophilic polymer
Particle Size (mean):	7 µm and 10 µm
Pore Size (mean):	non-porous
Functional Group:	carboxymethyl
Counter Ion:	Na⁺
pH Range:	3.0 - 10.0
Static Binding Capacity (mg Lysozyme/g dry gel):	ca. 20 (7 µm) ca. 15 (10 µm)
Small Ion Capacity:	100 µeq/g dry gel
pKa:	4.9
Hardware:	stainless steel tubing and fittings, PEEK frits

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0021965	TSKgel CM-STAT	10 µm	Stainless Steel	3	3.5
0021966	TSKgel CM-STAT	7 µm	Stainless Steel	4.6	10

Literature:

• Catalog

• IEC Column Products Table

Base Material:	hydrophilic polymer
Particle Size (mean):	5 µm
Pore Size (mean):	ND
Functional Group:	proprietary
Counter Ion:	H⁺
pH Range:	2.0 - 7.5
Small Ion Capacity:	> 1.5 eq/L

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0016653	TSKgel OApak-A	5 µm	Stainless Steel	7.8	30
0016654	TSKgel OApak-P Guard column	10 µm	Stainless Steel	6	4

Literature:

• Catalog

• IEC Column Products Table

• Instruction Manual

Base Material:	PS-DVB
Particle Size (mean):	5 µm
Pore Size (mean):	6.0 nm
Functional Group:	sulfonic acid
Counter Ion:	H⁺ and Na⁺
pH Range:	2 - 12
Small Ion Capacity:	> 1.5 eq/L

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0007156	TSKgel SCX (Na⁺)	5 µm	Stainless Steel	6	15
0007158	TSKgel SCX (H⁺)	5 µm	Stainless Steel	7.8	30

Literature:

• Catalog

• IEC Column Products Table

• Instruction Manual

Base Material:	spherical silica
Particle Size (mean):	5 µm
Pore Size (mean):	12.5 nm
Functional Group:	sulfopropyl
Counter Ion:	Na+
pH Range:	2.0 - 7.5
Protein Capacity	ND
Small Ion Capacity:	> 0.3 eq/L
pKa:	2.2

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0007165	TSKgel SP-2SW	5 µm	Stainless Steel	4.6	25
0007644	Guardgel Kit for P/N 0007165		Stainless Steel

Literature:

• Catalog

• IEC Column Products Table

• Instruction Manual

• Instruction Manual for Glass Columns

Base Material:	hydroxylated methacrylic polymer
Particle Size (mean):	10, 13, 20 µm
Pore Size (mean):	100 nm
Functional Group:	-(CH₂)SO₃^-
Counter Ion:	Na⁺
pH Range:	2.0 - 7.5
Protein Capacity :	40 g Hb/L
Small Ion Capacity:	> 0.1 eq/L
pKa:	2.3

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0018758	TSKgel SP-5PW	10 µm	Stainless Steel	2	7.5
0007161	TSKgel SP-5PW	10 µm	Stainless Steel	7.5	7.5
0007211	Guardgel Kit for P/N 0007161		Stainless Steel
0007575	TSKgel SP-5PW	13 µm	Stainless Steel	21.5	15
0016093	Guardgel Kit for P/N 0007575		Stainless Steel
0013062	TSKgel SP-5PW	10 µm	Glass	5	5
0008803	TSKgel SP-5PW	10 µm	Glass	8	7.5
0008807	Guardgel Kit for P/N 08803		Glass
0014017	TSKgel SP-5PW	13 µm	Glass	20	15
0014467	Guardgel Column for P/N 014017		Glass	20	2

Literature:

• Catalog

• IEC Column Products Table

• Instruction Manual

Base Material:	hydroxylated methacrylic polymer
Particle Size (mean):	2.5 µm
Pore Size (mean):	non-porous
Functional Group:	-(CH₂)SO₃^-
Counter Ion:	Na⁺
pH Range:	2.0 - 12.0
Protein Capacity :	5 mg Hb/mL
Small Ion Capacity:	> 0.1 eq/L
pKa:	2.3

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0013076	TSKgel SP-NPR	2.5 µm	Stainless Steel	4.6	3.5
0021942	TSKgel SP-NPR	2.5 µm	Stainless Steel	0.3	5

Literature:

• Separation Report No. 109

• Catalog

• IEC Column Products Table

• Instruction Manual

Base Material:	hydrophilic polymer
Particle Size (mean):	7 µm and 10 µm
Pore Size (mean):	non-porous
Functional Group:	sulfopropyl
Counter Ion:	Na⁺
pH Range:	3.0 - 10.0
Static Binding Capacity (mg Lysozyme/g dry gel):	ca. 15 (7 µm) ca. 10 (10 µm)
Small Ion Capacity:	23 µeg/g dry gel
pKa:	4.0
Hardware:	Stainless Steel tubing & fittings, PEEK frits

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0021963	TSKgel SP-STAT	10 µm	Stainless Steel	3	3.5
0021964	TSKgel SP-STAT	7 µm	Stainless Steel	4.6	10