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Polymer-Based RP Columns

Polymer-based RP Columns

Polymethacrylate-based reversed phase columns are available in a range of pore and particle sizes. Although often not as efficient and less robust than silica-based RPC columns, key advantages of polymer-based columns are:

Wider pH range (1 to 12) allows many basic compounds to be analyzed in their uncharged form, thus reducing secondary adsorption and improving peakshape.
Improved recovery for peptides and proteins due to reduced secondary interactions

TSKgel Octadecyl-NPR columns have a 2.5 µm particle size, fast kinetics, high column efficiency, and quantitative protein recovery at sub-µg loads. TSKgel Octadecyl-2PW is based on 5 µm particle size TSKgel G2000PW resin, with 12.5 nm pores, while the TSKgel Octadecyl-4PW is based on the TSKgel G4000PW resin, which contains 50 nm pores.

TSKgel Phenyl-5PW RP is based on 10 µm particle size TSKgel G5000PW resin, with an average pore size of 100 nm.

Literature:

• Catalog

• RPC Column Products Table

• Instruction Manual

Literature:

• Catalog

• RPC Column Products Table

• Instruction Manual

Particle Size (mean):	5 µm
Pore Size (mean):	12.5 nm
Molecular Weight Limit:	8,000 Da
pH Range:	2.0 - 12.0
Functional Group:	C18 (monomeric bonding chemistry)

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0018754	TSKgel Octadecyl-2PW	5 µm	Stainless Steel	2	15
0017500	TSKgel Octadecyl-2PW	5 µm	Stainless Steel	4.6	15
0017502	Guard Column for P/N 0017500	5 µm		4.6	1
0017501	TSKgel Octadecyl-2PW	5 µm	Stainless Steel	6	15
0017503	Guard Column for P/N 0017501	5 µm	Stainless Steel	6	1

Literature:

• Catalog

• RPC Column Products Table

• Instruction Manual

Particle Size (mean):	7 µm and 13 µm
Pore Size (mean):	50 nm
Molecular Weight Limit:	1,000 - 200,000 Da
Estimated Ligand Density:	1 meq/mL
pH Range:	2.0 - 12.0
Functional Group:	C18 (monomeric bonding chemistry)

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0018755	TSKgel Octadecyl-4PW	7 µm	Stainless Steel	2	15
0013351	TSKgel Octadecyl-4PW	7 µm	Stainless Steel	4.6	15
0019008	Guard Cartridge for P/N 4.6mm ID column, 3 pk, Replaces P/N 0014127	7 µm	Stainless Steel	3.2	1.5
0019018	Guard Cartridge Holder for P/N 0019008, Replaces P/N 0014100		Stainless Steel	3.2	1.5
0016257	TSKgel Octadecyl-4PW	13 µm	Stainless Steel	21.5	15

Literature:

• Catalog

• RPC Column Products Table

• Instruction Manual

Particle Size (mean):	2.5 µm
Pore Size (mean):	Non-porous
Molecular Weight Limit:	> 1,000,000 Da
Estimated Ligand Density:	1 meq/mL
pH Range:	2.0 - 12.0
Functional Group:	C18 (monomeric bonding chemistry)

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0014005	TSKgel Octadecyl-NPR	2.5	Stainless Steel	4.6	3.5

Literature:

• Catalog

• RPC Column Products Table

• Instruction Manual

Particle Size (mean):	10 µm and 13 µm
Pore Size (mean):	100 nm
Molecular Weight Limit:	1,000,000 Da
Estimated Ligand Density:	1 meq/mL
pH Range:	2.0 - 12.0
Functional Group:	Phenyl (monomeric bonding chemistry)

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0014007	TSKgel Phenyl-5PW RP	10 µm	Glass	8	7.5
0018756	TSKgel Phenyl-5PW RP	10 µm	Stainless Steel	2	7.5
0008043	TSKgel Phenyl-5PW RP	10 µm	Stainless Steel	4.6	7.5
0019007	Guard Cartridges for P/N 0008043, 3 pk, Replaces P/N 0014126	10 µm	Stainless Steel	3.2	1.5
0019018	Guard Cartridge Holder for P/N 0019007, Replaces P/N 0014100		Stainless Steel	3.2	1.5
0016260	TSKgel Phenyl-5PW RP	13 µm	Stainless Steel	21.5	15