TSKgel HHR column

TSKgel HHR Columns for Expanded Solvent Capability

The TSKgel HHR column line consists of eight columns with different pore sizes, TSKgel G1000HHR through TSKgel G7000HHR, and five columns with an extended linear range of the calibration curve. The calibration curves are shown below.

Main characteristics

Maximum operating temperature for TSKgel HHR-type columns is 140 ºC.
Broad solvent range. All TSKgel HHR-type columns are shipped in THF, which can be exchanged for a wide variety of organic solvents as shown below.
TSKgel HHR-type columns are conventional GPC columns with dimensions of 7.8 mm ID x 30 cm. Most HPLC systems do not require optimization of extra-column bandbroadening effects when working with conventional GPC columns.

Calibration Curves on TSKgel HHR Columns

Shipping Solvent

Can Be Replaced With

Tetrahydrofuran

acetone, ethanol, quinoline, benzene, o-dichlorobenzene, ethyl acetate,

dodecane, FC-113, carbon tetrachloride5, dichloromethane,

dichloroethane, trichloroethane, n-hexane, cyclohexane,

xylene, tetrahydrofuran, chloroform, 1,4-dioxane,

hexafluoroisopropanol, toluene, 1-chloronaphthalene,

dimethylaceto acetamide, methyl ethyl ketone, trichlorobenzene,

m-cresol, dimethylformamide, methylpyrrolidone,

o-chlorophenol/chloroform, dimethyl sulfoxide, pyridine

Notes:

In case of TSKgel SuperH and HHR, see table below for appropriate flow rates for solvent exchange. After switching to a very polar solvent, switching to a nonpolar is not recommended.
All TSKgel HXL, HHR, SuperHZ and SuperH analytical columns are shipped containing tetrahydrofuran (THF), except High Temperature (HT) columns, which contain o-dichlorobenzene.
Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.

* 100% methanol cannot be used with TSKgel H-type columns; use this solvent with TSKgel SW-type or Alpha-type columns.
** TSKgel H columns may be specially ordered with this shipping solvent.

Columns with linear calibration curves

The TSKgel HHR product line contains five linear, or mixed-bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The mixe-bed columns feature increasing linear calibration ranges, from TSKgel GMHHR-L, GMHHR-N, GMHHR-M, to GMHHR-H. TSKgel GMHHR-H(S) HT is a high temeperature mixed bed column shipped in ODCB, o-dichlorobenzene.

Solvent	Recommended Flow Rate
n-Hexane	0.9
methyl ethyl ketone	0.7
dichloromethane, ethyl acetate	0.6
toluene, chloroform	0.5
dimethylformamide	0.4
carbon tetrachloride, pyridine	0.3
dimethyl sulfoxide, dioxane, ethanol, N-methylpyrrolidone, o-dichlorobenzene	0.2
quinoline, hexafluoroisopropanal, 1-chloronaphthalene	0.1

Recommended Solvent	Application
THF	polystyrene, epoxy resin, phenoxy resin, polycarbonate, polyisoprene, polyvinyl acetate, polyvinyl chloride, monoglycerides, fatty acids, poly(butadiene), polybutadiene, poly(methyl methacrylate), poly(styrene-butadiene), poly(styrene-acrylonitrile)
N,N-Dimethylformamide (DMF) +/- 5mM LiBr	polyvinyl chloride, polyvinyl fluoride, urea resins, polyurethane, polystyrene, polyester, polyimido ether, polyimido ester, polyphenol (aqueous solution), polyacrylonitrile
o-Dichlorobenzene (ODCB)	polyethylene, polypropylene
Chloroform	polycarboxylic ether, acrylic resin, epoxy resin, polystyrene
m-Cresol/Chloroform	nylon, polyester, polyamide, poly(ethylene terephthalate)
Toluene	polybutadiene, polysiloxane

Literature :

• Separation Report No.83

• Catalog

• SEC Column Products Table

• Instruction Manual

Matrix:	polystyrene-divinylbenzene
Particle Size (mean):	5 µm
Pore size (mean):	1.5 nm
Exclusion Limit:	< 1,000 Da
Max. Temp. (ºC):	140
Shipping Solvent:	THF for 0017352 and 0017368 Chloroform for 0018270 and 0018271

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0017352	TSKgel G1000H_HR	5 µm	Stainless Steel	7.8	30
0017368	Guard Column for P/N 0017352	5 µm	Stainless Steel	6	4
0018270	TSKgel G1000H_HR	5 µm	Stainless Steel	21.5	30
0018271	Guard Column for P/N 0018270	5 µm	Stainless Steel	7.5	7.5

Matrix:	polystyrene-divinylbenzene
Particle Size (mean):	5 µm
Pore size (mean):	2 nm
Exclusion Limit:	< 10,000 Da
Max. Temp. (ºC):	140
Shipping Solvent:	THF for 0017353 and 0017368 Chloroform for 0018259 and 0018271

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0017353	TSKgel G2000H_HR	5 µm	Stainless Steel	7.8	30
0017368	Guard Column for P/N 0017353	5 µm	Stainless Steel	6	4
0018259	TSKgel G2000H_HR	5 µm	Stainless Steel	21.5	30
0018271	Guard Column for P/N 0018259	5 µm	Stainless Steel	7.5	7.5

Matrix:	polystyrene-divinylbenzene
Particle Size (mean):	5 µm
Pore size (mean):	3 nm
Exclusion Limit:	20,000 Da
Max. Temp. (ºC):	140
Shipping Solvent:	THF for 0017354 and 0017368 Chloroform for 0018266 and 0018271

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0017354	TSKgel G2500H_HR	5 µm	Stainless Steel	7.8	30
0017368	Guard Column for P/N 0017354	5 µm	Stainless Steel	6	4
0018266	TSKgel G2500H_HR	5 µm	Stainless Steel	21.5	30
0018271	Guard Column for P/N 0018266	5 µm	Stainless Steel	7.5	7.5

Matrix:	polystyrene-divinylbenzene
Particle Size (mean):	5 µm
Pore size (mean):	7.5 nm
Exclusion Limit	60,000 Da
Max. Temp. (ºC):	140
Shipping Solvent:	THF for 0017355 and 0017368 Chloroform for 0018267 and 0018271

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0017355	TSKgel G3000H_HR	5 µm	Stainless Steel	7.8	30
0017368	Guard Column for P/N 0017355	5 µm	Stainless Steel	6	4
0018267	TSKgel G3000H_HR	5 µm	Stainless Steel	21.5	30
0018271	Guard Column for P/N 0018267	5 µm	Stainless Steel	7.5	7.5

Matrix:	polystyrene-divinylbenzene
Particle Size (mean):	5 µm
Pore size (mean):	20 nm
Exclusion Limit:	< 400,000 Da
Max. Temp. (ºC):	140
Shipping Solvent:	THF for 0017356 and 0017368 Chloroform for 0018268 and 0018271

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0017356	TSKgel G4000H_HR	5 µm	Stainless Steel	7.8	30
0017368	Guard Column for P/N 0017356	5 µm	Stainless Steel	6	4
0018268	TSKgel G4000H_HR	5 µm	Stainless Steel	21.5	30
0018271	Guard Column for P/N 0018268	5 µm	Stainless Steel	7.5	7.5

Matrix:	polystyrene-divinylbenzene
Particle Size (mean):	5 µm
Pore size (mean):	65 nm
Exclusion Limit:	< 4 x 10⁶ Da
Max. Temp. (ºC):	140
Shipping Solvent:	THF

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0017357	TSKgel G5000H_HR	5 µm	Stainless Steel	7.8	30
0017369	Guard Column for P/N 0017357	5 µm	Stainless Steel	6	4

Matrix:	polystyrene-divinylbenzene
Particle Size (mean):	5 µm
Pore size (mean):	ND
Exclusion Limit:	< 4 x 10⁷ Da
Max. Temp. (ºC):	140
Shipping Solvent:	THF

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0017358	TSKgel G6000H_HR	5 µm	Stainless Steel	7.8	30
0017369	Guard Column for P/N 0017358	5 µm	Stainless Steel	6	4

Matrix:	polystyrene-divinylbenzene
Particle Size (mean):	5 µm
Pore size (mean):	ND
Exclusion Limit:	4 x 10⁸ Da
Max. Temp. (ºC):	140
Shipping Solvent:	THF

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0017359	TSKgel G7000H_HR	5 µm	Stainless Steel	7.8	30
0017369	Guard Column for P/N 0017359	5 µm	Stainless Steel	6	4

Matrix:	polystyrene-divinylbenzene
Particle Size (mean):	5 µm
Pore size (mean):	ND
Exclusion Limit:	< 4 x 10⁸ Da
Max. Temp. (ºC):	140
Shipping Solvent:	THF for 0017360 and 0017369 Chloroform for 0018269 and 0018272

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0017360	TSKgel GMH_HR-H	5 µm	Stainless Steel	7.8	30
0017369	Guard column for P/N 0017360	5 µm	Stainless Steel	6	4
0018269	TSKgel GMH_HR-H	5 µm	Stainless Steel	21.5	30
0018272	Guard column for P/N 0018269	5 µm	Stainless Steel	7.5	7.5

Matrix:	polystyrene-divinylbenzene
Particle Size (mean):	5 µm
Pore size (mean):	mixed
Exclusion Limit:	< 4 x 10⁶ Da
Max. Temp. (ºC):	140
Shipping Solvent:	THF

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0017392	TSKgel GMH_HR-M	5 µm	Stainless Steel	7.8	30
0017369	Guard Column for P/N 0017392	5 µm	Stainless Steel	6	4

Matrix:	polystyrene-divinylbenzene
Particle Size (mean):	5 µm
Pore size (mean):	mixed
Exclusion Limit:	< 400,000 Da
Max. Temp. (ºC):	140
Shipping Solvent:	THF

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0018055	TSKgel GMH_HR-N	5 µm	Stainless Steel	7.8	30
0017369	Guard Column for P/N 0018055	5 µm	Stainless Steel	6	4

Matrix:	polystyrene-divinylbenzene
Particle Size (mean):	5 µm
Pore size (mean):	mixed
Exclusion Limit:	4 x 10⁶ Da
Max. Temp. (ºC):	140
Shipping Solvent:	THF

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0017362	TSKgel GMH_HR-L	5 µm	Stainless Steel	7.8	30
0017369	Guard Column for P/N 0017362	5 µm	Stainless Steel	6	4

Matrix:	polystyrene-divinylbenzene
Particle Size (mean):	13 µm
Pore size (mean):	mixed
Exclusion Limit:	< 4 x 10⁸ Da
Max. Temp. (ºC):	140
Shipping Solvent:	ODCB

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
0018393	TSKgel GMH_HR-H(S) HT	13 µm	Stainless Steel	7.8	30
0018397	Guard column for P/N 0018393	13 µm	Stainless Steel	7.5	7.5

TSKgel HHR Columns for Expanded Solvent Capability

Calibration Curves on TSKgel HHR Columns

Shipping Solvent

Can Be Replaced With

Solvent

Recommended Flow Rate

Recommended Solvent

Application